脂多糖诱导小鼠腹腔巨噬细胞TNF-αmRNA、iNOSmRNA的表达

Effects of lipopolysaccharide induction on tumor necrosis factor-a and inducible nitric oxide synthase mRNA in mice peritoneal macrophages

目的观察脂多糖对小鼠腹腔巨噬细胞肿瘤坏死因子-α以及诱导型一氧化氮合酶表达水平的影响。方法按常规方法获取小鼠腹腔巨噬细胞,随机分成两组:空白对照组及LPS组(分别含LPS 0.01,0.1,1.0,10mg/L)。采用荧光定量PCR方法测定肿瘤坏死因子-α及诱导型一氧化氮合酶的基因表达情况。结果经0.01μg/mL LPS刺激后细胞TNF-αmRNAi、NOS mRNA表达水平均明显升高,与空白对照组比较有显著性差异(P<0.01),并且在一定范围内呈剂量-效应关系。结论LPS可诱导小鼠巨噬细胞TNF-αmRNA i、NOS mRNA的表达。

Objective To observe the effects of lipopolysaceharide （LPS） induction on tumor necrosis faetor-a （TNF-α） and inducible nitric oxide synthase（iNOS）mRNA in mice peritoneal macrophages.Methods The ceils were divided into two groups randomly： control group （cells incubated without LPS）, and LPS group （cells cultured in lnedium containing LPS with 0.01, 0.1, 1.0, 10mg/L respeetively）,then cells were harvested after 24h incubation. The TNF-α and iNOS gene expression were detected by real-time reverse transcription-polymerase chain reaction （real time-PCR）.Results The result analysis showed the expression of both iNOS mRNA and TNF-α mRNA in cells from 0.1mg/L LPS group was significantly increased compared with control group （P〈0.05）. Conclusions LPS can significantly increase the expression of iNOS at gene level.