微分离方法原代培养大鼠肾近曲小管上皮细胞

Primary culture of renal proximal convoluted tubular epithelial cells in rats by microdissection

目的建立比较理想的大鼠肾近曲小管上皮细胞的原代培养及鉴定方法。方法采用显微微分离单根肾近曲小管节段的方法进行原代培养,并用免疫细胞化学方法和电镜进行鉴定。结果用微分离方法成功地培养出肾小管上皮细胞,经鉴定为该细胞。结论微分离方法是大鼠肾近曲小管上皮细胞原代培养的有效方法。

Objective To establish a practical method for primary euhure and identification of rat renal tubular epithelial cells. Methods Primary culture of renal proximal convoluted tubular epithelial cells by microdissection. The cell types were idenfied by immunocytochemistry and electron mieroseope. Results The method for primary cuhure was successful, so was the identification of the cultured cells. Conclusions The present method is an efficient means for the primary culture and identification of rat renal tubular epithelial cells.