低氧培养促进小鼠骨髓间充质干细胞的增殖

Effects of Low Oxygen Tension on Proliferation of Murine Bone Marrow Stem Cells

目的小鼠骨髓间充质干细胞(Murine bone marrow stem cells,mBMSCs)体外培养增殖相对缓慢,细胞表型会逐渐丧失。本研究采用低氧培养观察mBMSCs的增殖和表型维持情况,以期寻找一种更简便和快速扩增mBMSCs的方法。方法用低氧浓度(4%O_2)和常规氧浓度(20%O_2)培养、扩增mBMSCs,观察比较两种氧浓度培养的细胞增殖速度、细胞形态和细胞周期分析等。结果低氧浓度(4%O_2)促进mBMSCs增殖,同代次细胞倍增时间明显短于常规氧浓度(20%O_2)细胞;相比低氧浓度(4%O_2),常规氧浓度(20%O_2)培养的细胞更容易发生去分化。在细胞周期分析中,低氧浓度培养的mBMSCs处于复制期的比例高于常规氧浓度培养细胞的比例。结论低氧(4%O_2)培养可以促进mBMSCs增殖,是一种简便的体外迅速扩增mBMSCs的较好方法。

Objective The proliferation of murine bone marrow stem ceils （mBMSCs） is relatively slowly in vitro and gradually lose their phenotype. To study the effect of low oxygen tension on proliferation of mBMSCs in order to find a better way for mBMSCs quick expansion. Methods Murine bone marrow stem cells were isolated and cultured at low oxygen tension （4% O2） or conventional oxygen tension （20% O2）. Their proliferation, cell morphology and DNA cycle were observed and compared. Results Low oxygen tension （4% O2） promoted the mBMSCs proliferation, and the cell doubling time （4% O2） culture was shorter than that of 20% O2. There were significant cell morphological differences between mBMSCs cultured at 4% O2 and 20% O2. Moreover, the portion of cells at 4% O2 in DNA synthetic period is higher comparing with 20% O2. Conclusion Low oxygen tension （4% O2） culture can promote mBMSCs proliferation and it may be a better way to expand mBMSCs in vitro for tissue engineering.