摘要
目的探讨蛇毒神经生长因子(vNGF)对大鼠脑缺血再灌注的保护作用。方法选用体重220～280g的健康雄性Wistar大鼠,分为假手术组、对照组及vNGF组(再分为25U、50U、100U3个剂量组)。所有大鼠侧脑室注药7d后处死。大鼠模型分别观察:按Longa法进行神经功能评分;免疫组化法检测GAP-43的表达。结果神经功能评分:假手术组神经功能评分为0分,其余各组均出现神经功能缺损,vNGF组大鼠评分低于对照组(P<0.05)。免疫组化:除假手术组外各组GAP-43均有表达。vNGF组的表达较对照组高(P<0.05)。vNGF组25U组GAP-43表达增加,50U组表达继续增高,100U组表达最高。结论在大鼠大脑中动脉缺血再灌注损伤后,vNGF能增强GAP-43的表达,对神经细胞有明显的保护作用。
Objective To investigate the cerebral ischemia/reperfusion protection mechanism of viper venom nerve growth factor（vNGF） by the change of expression of growth associated protein-43 （GAP-43） and neurological function.Methods 45 adult male Wistar rats （weight 220～280 g） were divided randomly into 3 groups：sham group（S,n=9）,balanced salt solution group （BSS,n=9） and venom nerve growth factor group （vNGF,n=27）.Each group was observed for 7 days.vNGF group was divided into 25 U,50 U and 100 U subgroups respectively.The following indexes in 3 groups were observed respectively：neurologic deficits and the expression of GAP-43 （immunohistochemistry method）.Results Neurological function：The scores of neurological function was 0 in S group.The neurological deficits score was lower at the same time in vNGF group than that in BSS group （P〈0.05）.Immunohistochemistry：GAP-43 expressed in both BSS group and vNGF group.The expression of GAP-43 in vNGF group increased in 25 U,and to maximum in 100 U.The expression of GAP-43 in BSS group was significantly lower than in vNGF group （P〈0.05）.Conclusion vNGF can effectively enhance and prolong the expression of GAP-43,increase the survival rats of nerve cells,and has the protection effect on nerve cells after cerebral ischemia injured.
出处
《中国康复理论与实践》
CSCD
2009年第12期1104-1107,F0003,共5页
Chinese Journal of Rehabilitation Theory and Practice
基金
广西自然科学基金(桂科自0728133)
广西医科大学博士后流动站基金(20702011046)
