微孔草子油对大鼠胚胎毒性和致畸作用研究

Embryo Toxicity and Teratogenic Effects of Microula Seed Oil on Rat

目的探讨微孔草子油对大鼠胚胎毒性和致畸作用。方法性成熟Wistar大鼠,雌性100只,雄性50只,按雌性:雄性=2:1合笼配对,每日清晨检查阴拴和精子,发现者定为受孕零天。孕鼠随机分为5组(每组20只):给药组(设3个剂量组:给予微孔草子油2.5g/kg、5.0g/kg、10.0g/kg,ig)、环磷酰胺组(7mg/kg,sc)和菜子油组(给予等容积菜子油,ig)。受孕第7天开始,给药组、菜子油组每天给予受试物1次,连续10d。环磷酰胺组于受孕第11天给予环磷酰胺,每日1次,连续3d。受孕第20天处死孕鼠,观察受试物对孕鼠和胚胎的毒性及致畸作用。结果微孔草子油各剂量组孕鼠体重、活胎率明显高于环磷酰胺组(P<0.01),死胎率、吸收胎率显著低于环磷酰胺组(P<0.01),与菜子油组间无显著性差异(P>0.05);微孔草子油各剂量组胎鼠体重、身长、尾长与菜子油组无显著性差异(P>0.05),外观、内脏、骨骼未发现畸形;环磷酰胺组140只胎鼠中有112只出现畸形。结论微孔草子油在2.5g/kg、5.0g/kg、10.0g/kg剂量时对孕鼠和胚胎无明显的毒性和致畸作用。

Objective To investigate toxicity and teratogenic effect of Microula seed oil on embryo of rat.Methods 150 mature Wistar rats （100 females,50 males） were selected with female∶ male = 2∶1 cage match.During the daily morning examination the sperm was discovered in the vagina as the zero day for conception.100 pregnant rats were randomly divided into 5 groups （n=20 in each group）：treatment group （included 3 groups：to give Microula seed oil 2.5 g /kg,5.0 g /kg,10.0 g /kg body weight,ig,respectively）,cyclophosphamide group（7 mg/kg body weight,sc） and the edible oil group （to give dose,such as volume as Microula seed oil,ig）.From the 7th day of pregnancy,the treatment group,the edible oil group were given intervention once a day for 10 days.From the 11th day of pregnancy cyclophosphamide group was given cyclophosphamide as intervention once a day,for 3 days.On the 20th day the pregnant rats were killed.Results The body weight of pregnant rats and the rate of live births were significantly higher in the Microula seed oil dose group than in the cyclophosphamide group （P〈0.01）,stillbirth rate and birth rate of absorption was significantly lower in the Microula seed oil dose group than in the cyclophosphamide group （P〈0.01）,and no significant difference from the edible oil group （P〉 0.05）;the fetal rat,body weight,body length,tail length in all groups of Microula seed oil was no significant difference from the edible oil group （P〉 0.05）.There was no malformation in appearance,viscera,bones in the treatment group and the edible oil group while there were 112 fetal rats with deformity in 140 in the cyclophosphamide group.Conclusion Microula seed oil at doses of 2.5 g /kg,5.0 g /kg,10.0 g /kg body weight had no significant role in the toxicity and teratogenicity on embryos of pregnant rats.