摘要
采用正交实验的方法,以芍药苷为考察目标,用反相高效液相色谱法(HPLC)测定复方中药中芍药苷的含量,考察了乙醇浓度、乙醇用量、提取时间和提取次数4个因素。结果表明,最佳提取工艺为A2B1C1D3,即80%的乙醇8倍量,回流提取3次,每次1小时。
By orthogonal experimental design, the content of Peoniflorin in compound Chinese herbal medicine is measured by reversed-phase high-performance liquid chromatography. Alcohol concentration, solvent amount, extraction times and extraction time as factors are analyzed. The results show that the optimum extracting condition is A2B1C1D3 , that was 80% alcohol, eight times amount,refluxing for 3 times and 1 h each time.
出处
《南阳理工学院学报》
2009年第4期64-66,70,共4页
Journal of Nanyang Institute of Technology
基金
南阳市科技局项目(2007S1310)
关键词
正交实验
复方中药
芍药苷
反相高效液相色谱法
Orthogonal experimental design
Polypharmacy of Chinese herbal medicine
Paeoniflorin
Reversed-phase high-performance liquid chromatography
