对从甘蔗根部分离的固氮成团泛菌的研究

Study on A Nitrogen Fixing Pantoea Agglomeras Isolated from the Root of Sugar Cane

从甘蔗根部分离的固氮菌株Ｗ１１，经细菌自动鉴定系统根据９６个生化试验指标，鉴定为成团泛菌。该菌株耐酸（ｐＨ３．５），耐高蔗糖浓度（３０％），产酸使培养基的酸度降低到ｐＨ４．４１，３．１ｍｍｏｌＮＨ４ＮＯ３或５８．８ｍｍｏｌＮａＮＯ３能抑制固氮酶活性。在培养基的蔗糖浓度为１０％，谷氨酸钠０．２５ｇ／Ｌ，ｐＨ５．５，２８～３０℃条件下，该菌株生长最佳，固氮酶活性最高。在耐高蔗糖浓度，耐酸，碳源利用。

A strain of nitrogen fixing bacteria, W11, isolated from the root of sugar cane has been identificated as Pantoea agglomeras using an auto identification system for bacterium based on 96 biochemical test data. W11 has the properties of tolerance to acid(pH3.5) and high sucrose concentration (30%), A amount of acid was formed and reduced the acidity of medium to pH of 4.41, the nitrogenase activity was inhibited in presence of 3.1mmol NH 4NO 3 or 58.5mmol NaNO 3. The optimum growth and maximum nitrogenase activity of W11 occurs at the medium containing 10% sucrose and 0.25g/L sodium glutamate, pH5.5,28～30℃. Its behaviours of tolerance to acid and high sucrose concentration, acid production and the use of carbon substrates is simiiar to that the Acetobacter diazotrophicus has. The strain W11 was inferred to have a nitrogen fixing relationship associated with the root of sugar cane.