PET纤维上SuMA的紫外光接枝及对蛋白质的固定化

The UV Induced Grafting of SuMA onto PET Fiber and Its Application of Immobilization of Protein

通过使用ＮａＩＯ４而不需除氧的条件下，利用紫外光接枝技术，成功地将ＳｕＭＡ或ＳｕＭＡ和ＨＥＭＡ二元单体共接枝到ＰＥＴ纤维上。在相同ＳｕＭＡ单体浓度下，ＨＥＭＡ的加入能显著提高ＳｕＭＡ的接枝率。如所期望的那样，接枝在ＰＥＴ纤维上的ＳｕＭＡ，在近中性的水溶液中，通过其活性酯基与蛋白质的—ＮＨ２反应，成功实现了对胰蛋白酶和肝炎表面抗体的固定化；ＨＥＭＡ的加入虽然提高了ＳｕＭＡ的接枝率，但是ＨＥＭＡ接枝产生的空阻却显著减少了固定的蛋白质量。

The monomer of SuMA or SuMA and HEMA was grafted onto the PET fibers successfully under UV irradiation by the use of NaIO 4 instead of the degassing process. Under the same concentration of SuMA, the use of HEMA could enhance the grafting yield of SuMA onto PET fibers obviously comparing with the process which only SuMA was used. As expected, proteins of trypsin and hepatitis surface antibody were immobilized onto the SuMA grafted PET fibers successfully under the neutral aqueous solutions, which was realized through the reaction between the active ester group of SuMA and amino group of protein. Although the use of HEMA during UV grafting could improve the grafting yield of SuMA onto PET fibers, the special impede on the couple reaction of SuMA and proteins caused by the grafted HEMA on PET remarkably inhibited the immobilization of protein, made the amount of immobilized protein lower than that of the only SuMA grafted samples.