摘要
按照ＲＨＬｃＤＮＡ核苷酸序列所预示肽链的氨基酸序列，化学合成了与肽链Ｎ端、Ｃ端和中部相对应的３个肽段ＨＬ１、ＨＬ２和ＨＬ３，并将它们分别偶联上载体蛋白ＫＬＨ，以偶联蛋白为免疫原免疫家兔，获得了抗血清。经ＥＬＩＳＡ检测表明，抗血清的滴度为１：５１２００～１０２４００。用所制备的抗血清进行Ｗｅｓｔｅｒｎ印迹分析。
According to rat hepatic lipase (RHL) cDNA nucleotide sequence and the predicted amino acid sequence, 3 polypeptides that correspond to amino,carboryl termini and middle part of RHL were chemically synthesized, coupled to carrier protein keyhole limpet hemocyanin(KLH) respectively and then used as immunogens in rabbits. Antisera were raised and the titer (1∶51200～102400) were detectable by ELISA. The antisera were applied to check the existing of RHL and their gene expression product by western blotting.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第6期759-764,共6页
Acta Scientiarum Naturalium Universitatis Pekinensis
关键词
肝脏
脂酶
抗血清
RHL
生化药物
动脉粥样硬化
synthetic polypeptide
rat hepatic lipase
Western blotting
antisera
expression product
