肿瘤坏死因子-α、白介素-6、丙二醛、超氧化物歧化酶在大鼠腹腔海水浸泡伤中的表达

The levels of tumor necrosis factor-α,interleukin-6,malondialdehyde and superoxide dismutase in rats with open celiac seawater immersion wounds

目的:探讨肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白介素6(IL-6)、丙二醛(MDA)及超氧化物歧化酶(SOD)在大鼠腹部开放合并海水浸泡中的表达。方法:采用腹部开放伤合并海水浸泡大鼠致伤模型。在光镜下观察肠黏膜损伤程度;利用酶联免疫吸附测定(ELISA)试剂盒检测血浆TNF-α、IL-6含量的变化;分光光度法检测小肠组织MDA、SOD的含量。结果:腹部开放伤合并海水浸泡后,大鼠小肠病理学检查出现小肠充血、淋巴细胞浸润及肠上皮细胞间紧密连接破坏等改变;血中TNF-α、IL-6水平升高(P<0.05或P<0.01);肠组织MDA水平升高,SOD活性降低(P<0.05或P<0.01)。结论:腹部开放合并海水浸泡可致大鼠肠黏膜屏障功能受损,TNF-α、IL-6、氧自由基参与了腹部开放海水浸泡伤后继发性炎症反应过程。

Objective To investigate the levels of tumor necrosis factor-alpha（TNF-α）,interleukin-6（IL-6）,malondialdehyde（MDA） and superoxide dismutase（SOD） in rats with open celiac seawater immersion wounds.Methods Fifty Wistar rat models of open celiac wound were randomly divided into five groups：normal control group（group A n = 10）,operation group（group B n = 10）,abdominal seawater immersion group（group C n = 10）,celiac physiological saline immersion group（group D n = 10） and celiac seawater immersion group（group E n =10）.Pathomorphological changes of intestine were observed under light microscope.The levels of plasma TNF-α and IL-6 were measured by enzyme-linked immunosorbant assay.The levels of MDA and SOD in intestinal mucosa were also measured.Results Intestinal congestion,lymphocytic infiltration and tight junction damage were observed in group E.The levels of TNF-α,IL-6 and MDA were increased,with decreased SOD in group E（P 〈0.05 or P〈0.01）.Conclusion Celiac seawater immersion can damage intestinal mucosal barrier in rats with open celiac seawater immersion wounds.TNF-A,IL-6 and reactive oxygen species（ROS） may be associated with the inflammatory reaction.