喉鳞状细胞癌中C-Met基因和细胞核增殖抗原的表达及其临床意义

Expression and significance of C-Met and proliferating cell nuclear antigen(PCNA) in laryngeal squamous cell carcinoma

目的:探讨喉鳞状细胞癌中C-Met和增殖细胞核抗原PCNA(proliferating cell nuclearantigen)的表达及其临床意义。方法:应用免疫组化SP法检测40例喉鳞癌中C-Met和PCNA的表达,并取30例声带息肉作对照。结果:C-Met在喉鳞癌中阳性表达率为57.5%,高于对照组6.7%(P<0.05)。C-Met在喉鳞癌中的表达与病理分级、临床分期及淋巴结转移有关(P均<0.05),与年龄、原发部位无显著相关(P>0.05)。PCNA在喉鳞癌中的表达率为77.5%,在声带息肉中为16.7%,两者间比较有显著差异(P<0.05)。PCNA的表达强度与喉癌的病理分级、临床分期及淋巴结转移呈显著相关,与年龄、原发部位无关。C-Met和PCNA的表达具有相关性(P<0.05)。结论:C-Met和PCNA的过表达在喉鳞癌的发生、发展及转移过程中发挥重要作用,二者对于判断喉癌的进展和预后具有重要的临床意义。

Objective To investigate the expression and significance of C-Met and PCNA in laryngeal squamous cell Carcinoma（LSCC）.Methods The expression of C-Met and PCNA was detected by immunohistochemical method in 40 cases of LSCC and thirty cases of polyp of vocal cord.Results The positive expression rate of C-Met in LSCC were 57.5%,which was significantly lower than that in normal control group（P〈0.05）.The expression of C-Met in LSCC was correlated with pathological stages,clinical stage and metastasis of lymph node（P〈 0.05）,but was not associated with age and tumor site（P〉 0.05）.The positive rate of PCNA was 77.5% and 16.7% in LSCC and polyp of vocal cord,respectively.PCNA expression was significantly related with the histological grade,clinical stage and metastasis of lymph node（P〈0.05）,but was not associated with age and tumor site.The C-Met activity was associated with PCNA expression in LSCC（P〈 0.05）.Conclusion The abnormal expression of C-Met and PCNA may be correlated with the development and metastasis of LSCC.The combined detection of C-Met and PCNA may be useful for evaluations of the progress and prognosis of LSCC.