螺旋藻片对小鼠睾丸染色体致畸变实验研究

Studies on the Impact of Spirulina on Chromosome Aberration in Testicle Cells of Mice

目的探讨螺旋藻片的毒性作用。方法以4.0、2.0、1.0g.kg-1BW(体重Body Weight)剂量的螺旋藻片连续给小鼠灌胃5d,取小鼠两侧睾丸,制备小鼠睾丸生殖细胞染色体标本。结果各剂量组小鼠睾丸染色体断片、易位、畸变细胞率、常染色体单价体、性染色体单价体与溶剂对照组比较无显著性差异(P>0.05),而阳性对照组断片、畸变细胞率、常染色体单价体、性染色体单价体与溶剂对照组比较有极显著性差异(P<0.01)。结论螺旋藻片各剂量组对小鼠睾丸生殖细胞无染色体畸变作用。

Objective To explore the toxic effects of Spirulina Tablets.Methods Give spirulina to mice through mouth under the dosage of 4.0,2.0,1.0g·kg-1BW by 5 days,and finally make chromosome slice of the testicle cells.Results There wasn＇t significant difference between the negative control group and the treated groups about the chromosome aberration ratio of the testicle cells（P〉0.05）,while significant difference was showed between the positive control group and the negative control group（P〈0.01）.Conclusions Spirulina does not induce chromosome aberration under the dosage of the test in testicle cells of mice.