拟除虫菊酯对昆虫 ATPase 的影响研究

The Effect of Pyrethroid on ATPase of Insect

美洲大蠊中枢神经系统ＡＴＰａｓｅ活力测定的最适反应条件是：对于Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ，蛋白质含量５～１０μｇ，ｐＨ值７．０，反应温度３０℃，反应时间１５ｍｉｎ．Ｋｍ和Ｖｍａｘ分别为５．３８×１０－３ｍｏｌ／Ｌ和１４３．７５ｎｍｏｌ／ｍｉｎ·ｍｇ－１．Ｃａ２＋－Ｍｇ２＋－ＡＴＰａｓｅ基本相同，只是ｐＨ值为７．４，Ｋｍ和Ｖｍａｘ分别为２．６４×１０－４ｍｏｌ／Ｌ和４６．２８ｎｍｏｌ／ｍｉｎ·ｍｇ－１．初步研究了氯氰菊酯及氰戊菊酯的不同异构体对ＡＴＰａｓｅ的影响，结果显示均对ＡＴＰａｓｅ有不同程度的抑制，与生测结果不完全一致．

The optimum reaction conditions of Na +K +ATPase and Ca 2+ Mg 2+ ATPase in central nervous system of Periplaneta americana are determined. For Na +K +ATPase the optimal protein concentration is 5～10μg, pH 7.0, temperature 30℃ and time 15 min. Km and Vmax are 5.38×10 3 mol/L and 143.75nmol/min·mg -1 , respectivey. The optimum conditions of Ca 2+ Mg 2+ ATPase are almost similar to those of Na +K +ATPase except that the pH 7.4. The Km and Vmax are 2.64×10 4 mol/L and 46.28nmol/min·mg -1 , respectively. Preliminary effect of pyrethroid on ATPase is studied. The results show that ATPase are inhibited by isomers of cypermethrin and fenvalerat, but it is no relation to that of KD 50 .