猪流行性腹泻病毒M蛋白B细胞表位预测分析

Prediction and Analysis of B Cell Epitopes for the M Protein of TGEV

目的分析TGEV M蛋白的B细胞表位,为试验确定TGEV M蛋白的B细胞表位和开发TGEV重组亚单位疫苗研究奠定基础;方法以TGEV M蛋白氨基酸序列为基础,分别采用Garnier-Robson、Chou-Fasman和Karplus-Schulz方法预测M蛋白的二级结构,以及利用在线TMHMM Server v2.0软件分析M蛋白的跨膜区;之后,分别按Kyte-Doolittle,Emini和Jameson-Wolf方案预测TGEV M蛋白的B细胞表位;结果预测结果表明,在TGEV M蛋白N端共同α-螺旋区段为4个;共同的β-折叠区段分别为13个;共同的转角区域分别为7个;柔性区域为12个;M蛋跨膜区3个;结论TGEV M蛋白N端第19～43、103～109、138～155、198～205、220～228和237～257区段内或附近很可能是B细胞表位优势区城。

Objective To identify the potential B cell epitopes of M protein of TGEV in theory,which is helpful for the development recombinant subunit vaccine of TGEV.Methods The secondary structure of M protein of TGEV and its transmembrane region were predicted by the methods of Garnier-Robson,Chou-Fasman,Karplus-Schulz and TMHMM Server v2.0 software online respectively, based on its amino acids sequence.And hydrophilicity plot,surface probability plot and antigenic index for M protein were analyzed by the methods of Kyte-Doolittle,Emini and Jameson-Wolf methods,respectively.Results There are 4 centers ofα-helix, 13 centers ofβ-sheet,7 turn regions,12 flexible regions,and 3 TMs in the M protein＇s N terminal,respectively.Conclusions Combined the previous results,the B cell epitopes for M protein of TGEV were predicted,which the B cell epitopes possibly localized in or nearby the M protein＇s N terminal No.19-43,103-109,138-155,198-205,220-228 and No.237-257.