从骨胶中提取L-羟基脯氨酸和L-脯氨酸

Extracting L-Hydroxyproline and L-Proline form Gelatin

从骨胶中提取L－羟基脯氨酸（L－HP）和L－脯氨酸（L－P），研究了胶原蛋白以L－HP为目的氨基酸的水解曲线；利用亚氨酸亚硝化的可逆性，从水解液中提取亚氨酸；用D61阳离子交换树脂层析分离L－HP和L－P；精制出L－HP和L－P晶体，得率分别为4．5％和4％。

The extraction Of L-hydroxypforine (L-HP) and L-proline (L-P) from gelatin has been studied. The hydrolysis of collagen with L-HP as the purpose amino acid was examined. Imino acid was extracted from hydrolysate by means of the invertibiliyty of indno arid nitrosylation and L-HP and L-P were separated by chromatography using D61 cation exchange resin. The purified L-HP and L-P were crystal and the yields were 4. 5% and 4% respctively.