中枢乙酰胆碱对体液免疫应答的影响

INFLUENCE OF CENTRAL ACETYLCHOLINE ON THE HUMORAL IMMUNE RESPONSE

以大鼠为实验动物,经绵羊红细胞免疫后,产生的抗体形成细胞初次反应的光密度值为指标,研究中枢乙酰胆碱对大鼠初次体液免疫应答的影响。结果表明:(1)侧脑室注射乙酰胆碱合成阻断剂密胆碱后,免疫应答增强。(2)侧脑室注射乙酰胆碱酯酶抑制剂新斯的明后,免疫应答抑制。(3)侧脑室注射乙酰胆碱的M型受体阻断剂,可增强免疫应答;而注射乙酰胆碱的N型受体阻断剂则抑制了免疫应答。提示中枢乙酰胆碱参与了体液免疫应答的调节,它的调节作用可能是通过其相应的受体实现的。

Using the value of optical density(OD) as the index to estimate the function of the antibody-forming cells in rats immunized with sheep red blood cells (SRBC), we investigate the influence of central acelylcholine(Ach) on the primary humoral immune response to SRBC in rat. The results were as follows: Hemicholinium-3 which inhibits the biosynthesis of acetylcholine, markedly enhanced the immune response when injected intracerebroventricularly (Icv). Neostigmine, the inhibitor of cholonesterase, suppressed the immune response when injected Icv. The immune response was increased following administration of atropine Icv, a muscarinic antagonist; but was suppressed after administration of hexamethonium, a nicotinic antagonist. These results suggest that Ach in the central nervous system can modulate the primary humoral immune response to SRBC in rat. Furthermore, the immunomodulation by central Ach may be mediated by its relevant receptors.