米曲霉高产酸性蛋白酶菌株的选育

Screening of acid protease-overproducing stranins of aspergillus oryzae

用2%的纤维素酶,35℃下水浴酶解3 h制得米曲霉部分去壁孢子,以它为诱变对象对其进行Licl-微波,DES-超声波复合诱变,并利用酪蛋白平板初筛,三角瓶固体培养测酶活复筛。最终选出1株高产酸性蛋白酶的菌株C2,其产酸性蛋白酶活力是原始菌株的135.0%。

The spores with partly cell wall of aspergillus oryzae were used as the experimental eaterial.The spores was incubated at 35 ℃ for 3 hours with 2% cellulose.Then Licl-Microwave,DES-Ultrasonic on the spores were carried out.Finally,one high acid-protease producing mutant C2 was screened out by casein-degradating plate experiments and calculated acid-protease activity.The acid-protease activity of C2 is 135.0% of the original strain.