摘要
用2%的纤维素酶,35℃下水浴酶解3 h制得米曲霉部分去壁孢子,以它为诱变对象对其进行Licl-微波,DES-超声波复合诱变,并利用酪蛋白平板初筛,三角瓶固体培养测酶活复筛。最终选出1株高产酸性蛋白酶的菌株C2,其产酸性蛋白酶活力是原始菌株的135.0%。
The spores with partly cell wall of aspergillus oryzae were used as the experimental eaterial.The spores was incubated at 35 ℃ for 3 hours with 2% cellulose.Then Licl-Microwave,DES-Ultrasonic on the spores were carried out.Finally,one high acid-protease producing mutant C2 was screened out by casein-degradating plate experiments and calculated acid-protease activity.The acid-protease activity of C2 is 135.0% of the original strain.
出处
《中国调味品》
CAS
北大核心
2010年第1期53-55,59,共4页
China Condiment
基金
四川省教育厅川菜发展研究中心资助(CC07Z05)
四川省自贡市科技计划项目资助(07GC015)
关键词
米曲霉
复合诱变
酸性蛋白酶
aspergillus oryzae
compound mutation
acid-protease