RP-HPLC同时测定白鲜皮中3种活性成分的含量

Simultaneous determination of three active compounds in root barks of Dictamnus dasycarpus by RP-HPLC

目的:建立同时测定白鲜皮中白鲜碱、黄柏酮和梣酮含量的反相高效液相色谱法,为中药白鲜皮标准的制定提供依据。方法:采用甲醇热回流提取,以Kromasil C18(4.6 mm×250 mm,5μm)为固定相,甲醇-水(60∶40)为流动相,检测波长236 nm,流速1 mL.min-1,柱温为25℃。结果:该3种成分分离良好,线性范围依次为0.002 1～0.106 0,0.020 1～0.920 0和0.010 2～1.020 g.L-1,加样回收率分别为100.5%,99.2%和100.2%。结论:本方法操作简单、快捷、重现性好、准确率高,可用于白鲜皮药材的质量控制。

Objective： To develop a RP-HPLC method for simultaneous determination of three active compounds, dictamnine, obacunone and fraxinellone from Dictamnus dasycarpus Turcz.. This will supply a reference for the establishment of the quality standard of D. dasycarpus. Method： A Kromasil C18 column was used with methanol-water （60∶40） as the mobile phase, at the flow rate of 1 mL·min^-1. 236 nm was selected as the detected wavelength. Result：The determined three compounds were well separated with a linear range of 0.002 1-0.106 0, 0.020 1-0.920 0 and 0.010 2-1.020 g·L^-1, respectively. The recoveries of them were 100.5 %, 99.2 % and 100.2 %, respectively. Conclusion： This method is simple, rapid and accurate, particularly suitable for the quality control of Dictamnus dasycarpus Turcz.