蟾蜕抗肿瘤及增强免疫效应研究

Studies on anti-tumor and enhancing immunity activity of toad coat

目的:研究一种新发现的蟾蜍药用部位——蟾蜕在小鼠体内的抗肿瘤及增强免疫效应的功能。方法:检测S180肉瘤、H22肝癌、Lewis肺癌荷瘤小鼠服用蟾蜕以后的肿瘤变化,观察蟾蜕延长HCA肝腹水癌小鼠寿命的效应,采用MTT法检测Lewis肺癌荷瘤小鼠服用蟾蜕以后的T淋巴细胞转化率和NK细胞活性变化,同时考察大剂量蟾蜕的服用是否对小鼠有明显毒性。结果:服用蟾蜕以后S180肉瘤、H22肝癌实体瘤、Lewis肺癌荷瘤小鼠肿瘤有明显缩小,Lewis肺癌荷瘤小鼠T淋巴细胞转化率和NK细胞杀伤活性均有明显上升,大剂量服用蟾蜕对小鼠无明显毒副作用。结论:提示蟾蜕在动物体内具有明显的抗肿瘤效果和增强免疫效应,是一种安全的可用于抗肿瘤治疗的药用动物新资源。

Objective： To study the anti-tumor and immunity activity of toad coat （ Chantui）, which is a new officinal part ofBufo bufo gargarizans. Method： The tumor weight of S180, H22, Lewis lung cancer cell inoculated in mice were compared between the groups of mice, fed with toad coat, and those which were not（ control group）. The average longevity of the mice with HCA fed with toad coat was also compared with the control group. The T lymphocyte transformation and NK cell killing activity were tested and compared with the control group. The condition of the mice which were fed with great dosage of Chantui（ 16 g · kg^-1· d^-1 ） was observed. Result： The tumor weight was remarkably reduced in the groups which were fed with toad coat compared with the control group. Tests show that toad coat can raise the activity of both T lymphocyte and NK cell There was no obvious side-effect when the mice were fed with great dosage of toad coat. Conclusion： The results show that toad coat has a strong inhibitory activity against tumors inoculated in mice and a strong enhancement of immune activity, so it could be viewed as a new valuable safe medicinal source.