实时荧光定量PCR检测健脾化瘀方对肝癌细胞ras/MAPK通路相关基因表达的影响

Effect of Jianpi Huayu Recipe on ras / MAPK pathway of human hepatoma cell

[目的]研究健脾化瘀方对人肝癌细胞ras/MAPK信号通路相关基因表达的影响。[方法]采用实时荧光定量核酸扩增检测系统(Real-time Quantitative PCR Detecting System,qPCR),比较药物作用后目的、基因表达水平的变化。[结果]ras/MAPK信号传导通路上的七个基因均有所降低,其中sos1和raf1基因表达降低的较为明显,与对照组相比差别有统计学差异。[结论]健脾化瘀方能抑制ras/MAPK信号通路上主要基因的表达,从而起到抑制肝癌细胞的生长,诱导细胞的凋亡。

[Objective]To study the effect of jianpi huayu recipe on ras/MAPK signaling pathway of human hepatoma cell. [ Methods ] Real - time quantitative PCR detecting system was used. [ Results ] Expression of seven genes depressed after drug action. Sosl and rafl gene＇s expression was obviously low,had significant difference. [ Conclusion] The mechanism of Jianpi Huayu Recipe maybe inhibits expression of genes on the ras/ MAPK signaling pathway.