参地降糖颗粒对胰岛素抵抗伴高血压模型大鼠胰岛素敏感性的影响

Effects of the Shendi Jiangtang Granule on Insulin Sensitivity in Insulin Resistance Accompanied Hypertension Model Rats

目的观察参地降糖颗粒对胰岛素抵抗伴高血压模型大鼠胰岛素敏感性的影响及其作用机制。方法6周龄雄性SD大鼠随机分为空白组、模型组、中药组。空白组给予正常喂养,其余大鼠给予高果糖餐喂养(FFR)6周建立胰岛素抵抗伴高血压模型。中药组给予4周参地降糖颗粒(3.24g.kg-1.d-1);各组每周测定血压,12周时以正常血糖高胰岛素钳夹法(GC法)对各组进行胰岛素敏感性指标M值的评价;检测下肢两侧比目鱼肌和上肢两侧长指伸肌中肿瘤坏死因子α(TNF-α)的浓度。结果参地降糖颗粒对FFR大鼠的M值有明显的提高作用(P<0.01);对骨骼肌组织中TNF-α有明显的降低作用(P<0.01)。结论参地降糖颗粒对FFR大鼠的胰岛素抵抗有显著的改善作用,其机制可能与降低骨骼肌组织中TNF-α含量有关。

Objective To observed the effect of Shendi Jiangtang Granule （Granule for reducing glucos）, on insulin sensitivity in insulin resistance accompanied hypertension model rats and its mechanism. Methods Six-week-old male Sprague-Dawley rats were divided into blank group, model group and Chinese medicine group. The blank group were fed with normal rat chow, and the others were fed with fructose-rich chow （FFR）. The Chinese medicine group was given Shendi Jiangtang Granule （3.24 g/kg/day）. Systolic blood pressure was measured weekly for 6 weeks. Then the euglycemic hyperinsulinemic glucose clamp technique was performed to estimate insulin sensitivity （M value）. At the end of the glucose clamp, the soleus and extensor digitorum longus （EDL） muscles were dissected out for determination of the role of tumor necrosis factor-α（TNF-α） by an ELISA assay. Results Treatment with Shendi Jiangtang Granule significantly improved the M value in FFR rats （P〈0. 01） ;and siginificanty lowered TNF-α levels in the skeletal museles（P〈0.01）. Conclusion Chinese medicine Shendi Jiangtang Granule may improve insulin resistance, the effect might be related to reducing TNF-α in the skeletal muscles in hypertensive and insulin resistant fructose-fed rats.