血清降钙素放射免疫分析

RADIOIMMUNOASSAY FOR THYROCALCITONIN IN SERUM

采用氯胺Ｔ法对人降钙素（ｈＣＴ，以下简称ＣＴ）进行１２５Ｉ标记，用ＳｅｐｈａｄｅｘＧ－５０柱分离纯化，得到比活度＞９．５ＴＢｑ／ｇ、放化纯度＞９５％、与过量抗体结合率为（８５—９１）％的１２５Ｉ－ＣＴ。建立了稳定的冻干系列标准及简便、快速的分析方法。标准曲线范围为８．８—３５１ｐｍｏｌ／Ｌ，在该范围内测定人血清的平均回收率为（９５．５—１０２．０）％，高浓度血清的稀释相关系数为ｒ＝０．９９９３—０．９９９８，灵敏度为５．３ｐｍｏｌ／Ｌ，批内变异系数为（３．２—８．６）％，批间变异系数为（４．７—１０．４）％，非特异结合率为（２．４—４．７）％，健康人血清ＣＴ含量的正常值为１１．７±６．４ｐｍｏｌ／Ｌ。

A specific radioimmunoassay(RIA) for thyrocalcitonin(CT) is developed and applied to the measurment of serum. CT is iodinated according to the method of ch T and purified by Sephadex G 50. The specific activity of 125 I CTB more than 9.5 TBq/g,the radiochemical purity is more than 95% and the excessive antibody binding is (85-91)%. The kit has a stable lyophilization standards. The range of detection is 8.8-351 pmol/L. This method is simple and fast. The average recovery in serum is (95.5-102.0)%.The intra and inter coefficients of variation are (3.2-8.6)% and (4.7-10.4)%,respectively. The nonspecific binding is (2.4-4.7)%,the sensitivity is 5.3 pmol/L and the correlation coefficient of high value serum dilution is 0.999 3-0.999 8.The content of CT in 82 healthy adults is 11.7±6.4 pmol/L.