^(35)S 标记抗人肝癌单克隆抗体Hepama-1的制备

PREPARATION OF 35 S MAb HEPAMA 1

采用Ｈ２３５ＳＯ４与苯胺合成３５Ｓ－苯胺硫酸盐，经高温转换成３５Ｓ－对氨基苯磺酸，然后再用碳二亚胺交联到抗人肝癌单克隆抗体Ｈｅｐａｍａ－１上。所得３５Ｓ－ＭＡｂＨｅｐａｍａ－１用Ｅｌｉｓａ方法检测仍保持良好的免疫活性。产品最高比活度可达５１．８ＧＢｑ／ｇ，放化纯度＞９５％，放化产率以Ｈ２３５ＳＯ４投料计算最高可达２７％，以３５Ｓ－对氨基苯磺酸投料计算最高可达４５％，适用于做荷瘤裸鼠动物模型放免治疗研究实验。

Monoclonal antibody Hepama 1(MAb Hepama 1) against human hepatoma is labelled with 35 S by the carbodiimine method. The 35 S sulfanilic acid is transferred from 35 S aniline sulfate which is synthesized by both H 2 35 SO 4 and aniline, and then, it is connected to the MAb Hepama 1. The resultant labelled MAb Hepama 1 retains immunoreactivity and had a maximum specific activity of 51.8 GBq/g and a radiochemical purity of >95%. The radiochemical yield are 27% and 45%, according to the primary activity of H 2 35 SO 4 and 35 S sulfanilic acid respectively. It is used as a radioimmunotherapy agent with a strong cytotoxic and tumorinhibitory effect in the Hepama bearing nude mice.