60例人类微小病毒B19感染患儿的病原血清学检测及特征

Diagnosis and clinical description of 60 pediatric cases of human parvovirus B19 infection

目的了解人类微小病毒Ｂ１９（ｈｕｍａｎｐａｒｖｏｖｉｒｕｓ，Ｂ１９）在儿童中的感染情况。方法采用聚合酶链反应（ＰＣＲ）和酶联免疫吸附（ＥＬＩＳＡ）方法，对１９４例住院治疗（大部分来自血液病房）患儿和１００例健康查体儿童的血清标本进行了检测。抗原为作者采用基因工程方法制备的重组Ｂ１９病毒外壳蛋白ＶＰ１和ＶＰ２。结果在１９４份患儿血清标本中，５５份检测出Ｂ１９病毒ＤＮＡ，３０份Ｂ１９病毒特异性ＩｇＭ抗体检测为阳性，３７份Ｂ１９病毒特异性ＩｇＧ抗体检测结果为阳性，阳性率分别为２８．４％，１５．５％和１９．１％，共有６０例患儿存在Ｂ１９病毒的近期感染。在１００份健康查体儿童血清标本中，３份检出Ｂ１９病毒ＤＮＡ，２份Ｂ１９特异性ＩｇＭ抗体检测结果为阳性，１２份Ｂ１９特异性ＩｇＧ抗体检测结果为阳性，阳性率分别为３０％，２０％和１２０％。结论人类微小病毒Ｂ１９在我国儿童中有较高的感染率，能够导致人类多种疾病。

Objective To investigate human parvovirus (HPV) B19 infection in children with a variety of clinical manisfestations. Methods A total of 194 serum speciemens from 194 hospitalized children (mostly in hematology ward) were tested with polymerase chain reaction (PCR) and enzyme linked immunosorbent assay (ELISA) and 100 healthy children served as controls for the tests. The antigens used in this study were recombinant HPV B19 capsid proteins (VP1 and VP2) produced in the laboratory of the authors. Results Of the 194 serum samples, 55 (28.4%) were found HPV B19 DNA positive by PCR; 30 (15.5%) were antiB19 IgM positive by ELISA and 37 (19.1%) were antiB19 IgG positive by ELISA. Totally 60 of the 194 pediatric cases had laboratory evidence of recent B19 infection. The control group had 3 (3.0%) HPV B19 DNA positive specimens; 2 (2.0%) antiB19 IgM positive and 12 (12.0%) antiIgG positive specimens, respectively. Conclusion Human parvovirus B19 is a common infection among children in our area and related to a broad spectrum of disorders. Therefore, great attention should be paid to the infection.