电聚合二氧化锆固载辣根过氧化物酶的第三代生物传感器的研制

Study of the third generation biosensor with electropolymerization zirconia to immobilize horseradish peroxidase

利用生物相容性良好的无机材料二氧化锆（ZrO2）对辣根过氧化物酶（HRP）进行直接固定，用交流阻抗法对修饰电极进行了表征，并用循环伏安法和计时电流法对过氧化氢（H2O2）生物传感器的性能进行了研究。结果表明，该法成功的制备了以电聚合ZrO：为载体的无介体第三代电流型生物传感器。其线性范围为1．00×10-6-1．87×10-3mol／L，线性回归方程为：ip（μA）=0．0023c（μmol／L）＋2．9324，相关系数r为0．9968，检测限为2．86×10-7mol／L。

A mediated-free third generation hydrogen peroxide based on the immobilization of horseradish peroxi- dase （HRP） on the biocompatible, inorganic materials zirconia（ZrO2） was developed. The modified process of the electrode was characterized by electrochemical impedance spectroscopy（EIS）. The analytical performance of the proposed biosensor toward H2O2 was investigated by cyclic vohammetry and chronoamperometry. The biosensor showed a linear response to H2O2 over a concentration range from 1.00 ×10-6 to 1.87 × 10-3 mol/L with the detection limit of 2.86 × 10-7 mol/L, the linear regression equation was/p （μA） = 0.002 3 c （μmol/L） ＋ 2.932 4 and the correla- tion coefficient was 0.996 8.