摘要
利用生物相容性良好的无机材料二氧化锆(ZrO2)对辣根过氧化物酶(HRP)进行直接固定,用交流阻抗法对修饰电极进行了表征,并用循环伏安法和计时电流法对过氧化氢(H2O2)生物传感器的性能进行了研究。结果表明,该法成功的制备了以电聚合ZrO:为载体的无介体第三代电流型生物传感器。其线性范围为1.00×10-6-1.87×10-3mol/L,线性回归方程为:ip(μA)=0.0023c(μmol/L)+2.9324,相关系数r为0.9968,检测限为2.86×10-7mol/L。
A mediated-free third generation hydrogen peroxide based on the immobilization of horseradish peroxi- dase (HRP) on the biocompatible, inorganic materials zirconia(ZrO2) was developed. The modified process of the electrode was characterized by electrochemical impedance spectroscopy(EIS). The analytical performance of the proposed biosensor toward H2O2 was investigated by cyclic vohammetry and chronoamperometry. The biosensor showed a linear response to H2O2 over a concentration range from 1.00 ×10-6 to 1.87 × 10-3 mol/L with the detection limit of 2.86 × 10-7 mol/L, the linear regression equation was/p (μA) = 0.002 3 c (μmol/L) + 2.932 4 and the correla- tion coefficient was 0.996 8.
出处
《化学传感器》
CAS
2009年第4期48-52,共5页
Chemical Sensors
基金
国家自然科学基金(20675064)
重庆市自然科学基金(CST-2004BB4149
2005BB4100)
高新技术培育基金(XSGX02)项目资助