p53和p16在胆囊癌中的表达

EXPRESSION OF p53 AND p16 IN GALLBLADDER CANCER

目的探讨抑癌基因ｐ５３和ｐ１６改变对胆囊癌发生的作用。方法采用免疫组化方法检测３４例胆囊癌和２６例慢性胆囊炎标本中ｐ５３和ｐ１６的表达。结果慢性胆囊炎标本中ｐ５３１例阳性，阳性率为３．９％。胆囊癌中ｐ５３有１７例阳性，阳性率为５０％。两者表达差异有显著性（Ｐ＜０．００１）。ｐ１６在慢性胆囊炎标本中有１４例阳性，阳性率为５３．８％，胆囊癌中２９例阳性，阳性率为８５．３％，明显高于慢性胆囊炎（Ｐ＜０．０２）。结论ｐ５３过表达与胆囊癌发生之间存在一定的联系，但对胆囊癌的发展、侵袭影响不大。ｐ１６在胆囊癌发生中可能受某些癌基因的反馈调节而过度表达，其作用机制尚不十分清楚。

Objective:The pathogenesis effect of tumor suppressive genes to gallbladder cancer was discussed. Methods:Immunohistochemical technique was used to detect the expression of p53 and p16 in paraffin embedded gallbladder cancer and in cholecystitis samples.Results:17 out of 26 cholecystitis cases (3.9%) and 17 out of 34 gallbladder cancer cases (50%) had p53 expression, P<0.001. p16 expression was shown in 14 out of 26 cases (53.8%) with cholecystitis and in 29 out of 34 (85.3%) gallbladder cancer cases,P<0.02.Conclusion:There was some relationship between p53 expression and pathogenesis of gallbladder cancer.p53 expression was not correlated with histologic grade and invasiveness of gallbladder cancer. p16 was overexpressed in gallbladder cancer,the exact mechanism was unknown, it may be due to feedback modified by some other oncogenes.