慢性阿片依赖者在戒断阶段细胞免疫功能的研究

Study on cell-mediated immune function of human beings with chronic opioid dependence during withdrawal period

为科学地鉴定、预防毒品相关死亡提供一定的实验证据，采用免疫组化、细胞培养、核素掺入、ELISA等方法，研究26例用药模式相近的慢性阿片依赖者在戒断阶段的细胞免疫功能，并与健康对照组进行统计比较。结果表明：慢性阿片依赖者戒断期CD＋4细胞数目减少（30．40±9．45％，P＜0．05），CD＋4／CD＋8比例降低（1．27±0．19，P＜0．01）；PBMC合成DNA的能力下降（SI＝8．62±3．20，P＜0．01），产生IL－2的水平降低（23．78±8．77，P＜0．06）和产生IL－6的能力呈降低趋势（468．81±196．40，P＞0．05）；血清中免疫抑制性因子sIL－2R水平显著升高（566．33±265．50，P＜0．01）。慢性阿片依赖者在戒断阶段有多种免疫参数紊乱，细胞免疫功能低下。

Immunohistochemistry (APAAP method ), cell culture, 3H- TdR and ELISA technique were used to study immune function of 26 human beings with chronic opioid dependence and 20 normal controls. The results showed that the cell immune suppression in expression of lossing CD+4 cell (P < 0.05 ), disturbing proportion of CD+4 /CD+8 (P <0. 01 ), suppressing PBMC proliferation (P < 0. 01 ), decreasing IL-2 (P < 0.05) and IL6 production (P >0. 05) and increasing the level of sIL-2R in serum (P < 0. 01 ) Were occured in case chronic opioid dependence during withdrawal period. Our results provide experimentul basis for preventing drug-re1ated death.