摘要
本文用BA-细胞酶联免疫吸附试验(cell ELISA)替代敏感性差、对不溶性抗原不适用的琼脂双扩散试验,用以检测兔抗大鼠肾小管刷状缘抗原(FX1A)的抗体滴度,并对影响检测效果的几种因素进行了探讨。结果表明:美商陆预先包板能增强细胞对酶标板结合能力,以60μg/ml的浓度最佳。大鼠皮质细胞以6×10~6/ml的浓度包被则达到检测的最高敏感度,如高于该浓度,并不能继续增加敏感性。高滴度的兔抗大鼠FX1A抗体,成功地应用于免疫组化,提示BA-cell ELISA用以对兔抗大鼠FX1A抗体的检测是一种敏感性强、特异性高的检测手段。
In order to detect rabbit antibody directed against rat renal tubular brush border antigen (FXIA), we used BA-cell enzyme-linked immunosorbent assay instead of double diffusion in gel that was insensitive and inapplicable to insoluble antigen and investigated several factors of influence on measuring effect. The result showed that phytolacca americana was useful as binding. The dose of 60μg/ml was the best. At cell densities above 6×10~6 per well,the sensitivity did not increase. The 6×10^(-8) per well rat cortex cells were chosen for routine coating of plates. A high titer of serum anti-FXIA antibody was used successfully in immunohistochemistry. BA-cell ELISA was found to be a sensitive, specific means of measuring rabbit anti-FXIA antibody.
出处
《南通医学院学报》
1990年第4期280-282,350,共3页
ACTA Academiae Medicinae Nantong
