摘要
目的:采用HPLC梯度洗脱法同时测定决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚的含量。方法:用Kromasil 100A C_(18)色谱柱(5μm,4.6mm×150mm,迪马公司);甲醇-0.1%磷酸溶液梯度洗脱:0~15min(65:35),15~16 min(65~85:35~15),16~40 min(85:15);柱温:室温;检测波长:285nm(0~15min),254nm(15~40min);流速:1mL·min^(-1)。结果:橙黄决明素、大黄素、大黄酚和大黄素甲醚的线性范围分别为0.00038~0.00608μg(r=0.9998)、0.0029~0.0464μg(r=0.9999)、0.00105~0.0168μg(r=0.9996)、0.00044~0.00704μg(r=0.9998),平均回收率分别为99.50%(RSD=0.71%)、99.59%(RSD=1.42%)、99.31%(RSD=0.59%)、99.74%(RSD=0.91%)。结论:本方法专属性强,重复性好,可用于决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚的含量测定。
This study aimed to simultaneously measure the content of aurantio-obtusin, emodin, chrysophanol and physcione and in semen cassiae by HPLC. A Kromasil 100A C18 column (5μm, 4.6mm×150mm, DIKMA) was used for the gradient elution with a methanol-0.1% phosphonic acid solution: 0-15min (65:35), 15-16min (65-85: 35-15), 16- 40min (85:15), at the flow rate of 1 mL·min^-1 at room temperature.The UV wavelength was detected at 285 nm (0- 15min) and 254nm (15-40 min). The linear range was determined to be 0.00038-0.00608μg (r=0.9998), 0.0029- 0.0464μg (r=0.9999), 0.00105-0.0168μg (r=0.9996) and 0.00044-0.00704μg (r=0.9998) respectively for aurantioobtusin, emodin, ehrysophanol and physcione, with the average recovery of 99.50% (RSD=0.71%), 99.59% (RSD= 1.42%), 99.31% (RSD=0.59%) and 99.74% (RSD=0.91%). The results showed that this method has good specificity and repeatability, and thus considered as a good tool for the measurement of aurantio-obtusin, emodin, chrysophanol and physeione in semen cassiae.
出处
《世界科学技术-中医药现代化》
2009年第6期868-871,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
科技部国家自然科技资源共享平台项目(2005DKA21000):药用植物种质资源标准化整理
整合及共享试点-子课题:"川牛膝
决明
小决明
穿心莲
大麻
赤小豆等
负责人:卫莹芳(总课题负责人:黄璐琦)