辅酶Q_(10)与供心缺血再灌注损伤

Coenzyme Q_(10) and Ischemia-reperfusion Injury of Donor Heart

目的：探讨Co－Q10与供心缺血再灌注损伤的关系，评价Co－Q10对供心缺血再灌注损伤的保护作用。方法：选用16只新西兰大白兔随机分为对照组和实验组。常规取心后用UW液冷保存6小时；然后在Langendorff模型上，对照组用KHB液灌注，实验组用KHB＋Co－Q10液灌注30分钟。分别测定灌注前、后的心肌丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。结果：实验组MDA含量明显低于对照组（P＜O．05），SOD活性明显高于对照组（P＜0．05）。结论：Co一Q10对供心缺血再灌注损伤具有保护作用，值得临床应用。

Objective: This study is to investigate the relation of Co-Q10 and ischemia reperfusion injury of donor heart;and valus the protective effect of Co-Q10 on ischemia reperfusion injury of donor heart.Mehtods: New-Zealand Rabbits (n =16) were divided into two groups:control group as group 1, expenmental group as group 2. After hearts were isolated,preservation in UW fluid for six hours was given.Furthermore,on Langendorff model, hearts in group 1 were reperfused with KHB fluid while hearts in group 2 with KHB+ Co- Q10 fluid for 30 minutes. The MDA MDA and SOD were determined before reperfusion and at the end of reperfusion. Results: The MDA in group 2 was lower than group 1, but the SOD was higher in group 2 (P<0.05).Conclusions: Co- Q10 has an obvious protective effect on ischemia-reperfusion injury of donor heart.