桑菊饮含药血清对小鼠巨噬细胞Toll样受体表达的影响

Effect of the Drug Serum Containing Sangjuyin on the Expression of Toll-like Receptors in RAW 264.7 Cells

目的：研究桑菊饮对小鼠肺巨噬细胞株RAW264.7表达Toll样受体（TLR）的影响。方法：用桑菊饮含药血清作用于正常RAW264.7细胞,24 h后以荧光定量PCR方法测定TLR-1-TLR-9 mRNA的表达,探讨桑菊饮对Toll样受体表达的影响。结果：桑菊饮含药血清在一定剂量（7.7 g.kg-1.U-1）时,可以促进TLR-4和TLR-7的表达,而对TLR-1,TLR-2,TLR-3,TLR-5,TLR-6,TLR-8和TLR-9的表达没有明显影响。结论：桑菊饮的药效学作用可能与促进Toll样受体某些亚型表达有关。

Objective： To investigate the influence of Sangjuyin drug serum on the expression of TLRs on RAW264.7. Methods： RAW264.7 cell line was stimulated with the drug serum containing Sangjuyin. Expressions of TLRs mRNA were detected with real-time PCR after 24 hours. Results： The serum contained Sangjuyin could upregulated the expression of TLR-3 mRNA and TLR-7 mRNA in RAW264.7 cells. Conclusion： Promoting the expression of Toll-like receptors mRNA may be the elementary basis of Sangjuyin to play the effect.