保利甘胶囊对酒精所致急性肝损伤的保护作用

Protective Effects of Baoligan Capsule on Acute Alcohol-induced Liver Injury in Mice

目的:研究保利甘胶囊对酒精所致肝损伤的保护作用。方法:观测保利甘胶囊(300及600 mg.kg-1)连续ig 7d,对ig酒精8 g.kg-1致急性肝损伤模型小鼠血浆丙氨酸氨基转移酶(ALT)活性,肝组织丙二醛(MDA)含量,荧光酶标仪测定肝组织抗氧化能力指数(ORAC),HPLC法测定谷胱甘肽(GSH)含量,谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(SOD)活性的影响。结果:与模型组相比,保利甘胶囊可以明显降低酒精负荷小鼠血浆ALT活性和肝组织MDA含量,有效提高肝组织的ORAC指数、GSH含量、GSH-Px和SOD活性。结论:保利甘胶囊对酒精所致的小鼠急性肝损伤有一定的保护作用。

Objective： To observe the protective effects of Baoligan capsule on acute alcohol induced liver injury in mice. Methods： After oral administration of Baoligan （300 and 600 mg·kg^-1） for seven days, the mice were administrated alcohol（8 g·kg^-1 ）to induce liver injury. Hepatic function was evaluated by assessing alanine aminotransferase （ALT） level in plasma. TBARS method was performed to detect the contents of malondialdehyde （MDA） in liver. Antioxidant capacity of liver was measured by oxygen radical absorbance capacity （ORAC） assay. The contents of glutathione （GSH） were detected in liver by HPLC method. Hepatic glutathione peroxidase （GSH-Px） and superoxide dismutase （SOD） were detected by enzymology method. Results： Baoligan capsule decreased the levels of ALT in plasma and the content of MDA in liver. It also increased the levels of ORAC, GSH, GSH-Px and SOD in liver as compared with alcoholic control group. Conclusion： Baotigan capsule exerted protective effects on liver injury in mice loaded with alcohol.