生物酶解法提取苦楝素工艺过程的研究

Study on extraction technology of toosendanin by enzymatic hydrolysis

采用国产纤维素酶64001131,研究生物酶解法提取苦楝树皮中苦楝素的工艺条件。优化的工艺条件为:酶解液pH值4.5,酶解温度55℃,酶解时间1.5 h,酶浓度0.15 mg/mL。在此条件下,苦楝素的提取率达2.008%。

The process of enzymatic hydrolysis extraction of toosendanin was optimized by experiments. The optimum extraction technology was determined as follows： pH value of the enzymatic solution 4.5, enzymatic hydrolysis at 55℃,1.5 h for enzymatic hydrolysis time,the amount of enzyme 0.15 mg/mL and the yield of toosendanin can up to 2.008%.