矮丛蓝莓‘北极星’启动培养研究

Studies on Primary Culture of V.angustifdium ‘Polaris’

以矮丛蓝莓栽培品种‘北极星’的带腋芽茎段和幼嫩叶片为外植体,对启动培养的几个影响因素进行了研究。结果表明:矮丛蓝莓组织培养的最佳取材季节是4月份,该时期外植体的污染率最低;带腋芽茎段最佳灭菌方式为75%酒精30 s+0.1%HgCl28 min;带腋芽茎段以WPM+6-BA 3 mg/L+NAA 0.2 mg/L诱导效果最佳,诱导率为72.5%;幼嫩叶片以WPM+6-BA 1 mg/L+NAA 0.1 mg/L+2,4-D 2 mg/L诱导效果最佳,出愈率为42.5%。

Stem with axillary bud and tender leaf of V. plants for the experiment to research several influence time of drawing material for V. angustifdium tissue angustifdium of one cultivated varieties Polaris＇were used as exfactors of primary culture. The results indicated that the optimal culture was April. During this period, the contamination rate of explant was the lowest; The optimal sterilizing treatment for the stem with axillary bud was 75% Alcohol 30 s＋0. 1% HgCl28 min; it was also found that the optimal primary medium for stem with axillary bud was WPM＋6-BA 3 mg/L＋ NAA 0. 2 mg/L, the induction rate was 72. 5%; The optimal primary medium for tender leaf was WPM＋6-BA 1 mg/L＋NAA 0. 1 mg/L＋2,4-D 2 mg/L,the induction rate was 42. 5%.