摘要
以6个灰树花菌株(灰分、灰通、灰怀、灰1、灰4和灰高)为材料,利用酯酶同工酶、RAPD和ISSR 3种分子生物学技术对其进行比较分析。结果表明:经酯酶同工酶经聚类分析,可将6个灰树花菌株分为三大类,灰分、灰通、灰怀和灰1为一类,灰4单独为一类,灰高单独为一类;经特异性较好的RAPD和ISSR引物验证,其结果与酯酶同工酶分析的一致。
Three molecular biology techniques of esterase isozyme electrophoresis, random amplified polymorphic DNA （RAPD） and inter-simple sequence repeat（ISSR）were used to analyze the genetic diversity of six Grifola frondosa strains. The results from the different bands analysis showed that the six Grifola frondosa strains were divided into three groups. Strains Huifen,Huitong,Huihuai and Huil were divided into one group. The strain hui4 and huigao was divided into one group,respectively. After being tested by special primers of RAPD and ISSR, the results of DNA level were same to esterase isozyme electrophoresis.
出处
《北方园艺》
CAS
北大核心
2010年第1期201-204,共4页
Northern Horticulture
基金
国家科技支撑计划资助项目(2007BAD89B07)
关键词
灰树花
酯酶同工酶
RAPD
ISSR
grifola frondosa
esterase isozyme electrophoresis
RAPD
ISSR
