纤维连接蛋白在胚胎着床前后子宫内膜组织中的动态表达

The Expression of Fibronetin in Endometrium before and after Embryo Implantation

为了探讨纤维连接蛋白(FN)在胚胎着床中的作用,本研究应用蛋白印迹和免疫印迹法检测小鼠孕3、5、7、15天子宫内膜及未妊娠小鼠子宫内膜组织中FN蛋白表达量,对免疫印迹法结果进行VDS扫描半定量分析后发现:与未妊娠小鼠相比,妊娠各期小鼠子宫内膜组织中FN蛋白表达量明显升高,其高峰在着床后的孕7天,直至孕15天.这一结果说明,在着床前,小鼠子宫内膜细胞外基质已发生变化,FN表达增高以利胚胎着床,而着床这一过程又加剧细胞外基质的变化,使FN在着床后表达到高峰,着床完成后,细胞外基质趋于稳定,FN又有所下降.本研究还用以上方法测定了人早孕胚囊平均直径在7～25mm(相当于孕35～49天)时的蜕膜组织中FN的表达,发现孕35～49天各期蜕膜组织中的FN表达量无差异,但均明显较未孕组高,说明人胚胎着床后,FN表达也明显增多,并到一定程度后趋于稳定.FN在着床前后的活跃变化说明它与着床有密切关系.

In order to research into the role of fibronectin (FN) in implantation of embryo,the FN pro-tein expression in endometrium of mice on the day 3,5,7,15 of gestation was detected with the methods of western blot and immunoblot. The endometrium from nonpregnancy was used as con-trol. The blots were scanned with VDS system. It was found that the expression of FN in en-dometrium of various periods of gestation of mice was much higher than that in nonpregnancy. On d3 gestation,the FN expression began to increase,then went up to the highest level on the d7 and till the d!5 of gestation. From those results it could be inferred that the FN contents of en-dometrium were changed to be ready to receive embryo before embryo was implanted, which stim-ulated the altering of FN in endometrium to optimize the embryonic development and also made FN expression maintain a higher level. Human decidual tissues during early pregnancy were also detected with the same methods. It resulted that when the diameter of embryonic sac was 7~25 mm (eguivalent to 35~49 days of gestation) ,FN protein was expressed in endometrium in about the same amount,but much more than that in nonpregnant endometrium. The increase of FN ex-pression may be related to embryo implantation. From the active changes of FN expression during implantation .we concluded that FN could play an important role in it.