FQ-PCR检测HPV基因型的临床应用分析

Clinical Study on Human Papillomavirus Detection and Genotyping by Fluorescent Quantitative-PCR

目的了解人乳头瘤病毒感染患者HPV6,11型和HPV16,18型的分布。探讨HPV-DNA分型检测的临床应用价值。方法采用荧光定量聚合酶链反应(FQ-PCR)方法检测1567例HPV感染者疣体组织或分泌物中的HPV6,11型和HPV16,18型。结果男女性均可感染HPV6,11和HPV16,18型,男性感染者以HPV6,11型为主,感染率为27.68%(157/567)。女性1000人次中,确诊感染HPV有659人,HPV阳性320人次,检出率为32%,其中低危型208例,即HPV6,11型阳性率为31.56%;高危型112例,即HPV16,18型为16.99%。结论FQ-PCR具有灵敏度高、特异性强、简捷方便的特点,适于临床推广应用。HPV DNA分型检测技术可以快速区分高危型及低危型HPV感染,有助于对尖锐湿疣的复发和宫颈癌变作出可能性预测。

Objective To study the HPV6,11 and HPV16,18 DNA infectious status of patients with human papillomavirus. To assess the value of HPV DNA genotyping in clinical application. Methods Fluorescence quantitative PCR （FQ-PCR） was used to measure HPV 6,11 and HPV 16,18 DNA in 1 567 wart tissues or secretion samples. Results No matter man or woman, all could be infected by HPV 6,11,16,18. Among these, the main subtype of man was HPV 6,11 and the positive rates of HPV 6,11 detection as 27.68%（157/567）. Altogether 320（32%） women were HPV positive among the 1 000 female patients. In 320 HPV positive cases,there were 208 cases of positive HPV 6,11,and 112 cases of positive HPV 16,18,respectively. In 659 female patient with condyloma acuminatum,the infection rate of HPV 6,11 accounted for 31. 56%（208/659）, the positive rate of HPV 16,18 DNA was 16. 99%（112/659）.Conclusion FQ-PCR is suitable for clinical application because of its high sensitivity,rapidity and accuracy. The technique of HPV DNA genotyping can quickly classify whether the infected HPV is high or low risk,which help to forecast the recurrence of condyloma acuminata and canceration of cervical cancer.