^(153)Sm标记抗TPS单克隆抗体在荷人乳腺癌裸鼠体内的预定位显像

Radioimmunoimaging study using pretargeting system on ^(153)Sm labeled anti-TPS monoclonal antibody in nude mice grafted with human breast carcinoma

目的以153Sm标记的抗TPS单克隆抗体对荷人乳腺癌裸鼠进行肿瘤预定位显像的研究。为乳腺癌早期诊断提供一种新方法,并为其临床应用提供实验依据。方法实验Ⅰ组每只裸鼠预定位法从尾静脉注射7.4MBq/0.1ml标记抗体。实验Ⅱ组与Ⅲ组分别直接从尾静脉注射7.4MBq/0.1ml的135Sm-TPS及135Sm-IgG。并于于注射后2、4、8、24h行全身SPECT显像。于2、4、8、24h分批处死裸鼠并测定分别在肿瘤、血液、心脏、肺等重要脏器单位重量的放射性比值(%ID/g)。结果在运用预定位技术加药的实验I组在给药后2小时开始有抗体在肿瘤组织浓聚,在8小时时达到最高,与实验II组直接给予抗体比较,血液本底低,两组比较差异有显著性。(P<0.05)。结论应用预定位技术的实验组,瘤/血液比值高,血液本底低,周围正常组织吸收剂量低,显像效果好。

Objective To study using pretargeting system on radioimmunoimaging of 153Sm labeled anti-TPS mouodonal antibody and normal mice IgG in nude mice with human breast carcinoma and provide the basis for clinical use. Methods group I 3-step pre- targeting injected into the tail vein with 7.4 MBg/0.1 ml labeled antibody, group Ⅱ and Ⅲ injected directly into the tail vein with 7.4 MBg/0.1 ml labeled antibody. SPECT were measured after 2,4,8,24 h interval of injecion. Percent injected dosage of every gram tissue（ %ID/g）were measured after 4,8,24 h interval to death.Results The tumor to blood ratio reached 7.36,9.22,11.11 at 2, 4,8 hours respectively. Radioactivity concertratoin peaked at 8 h in tumor site. the tumor/blood was higher than 153Sm-IgG（ P 〈 0.05）. Conclusion pretargeting system on radioimmunoimaging improves the amounts of locatingon toumors and yields a specific en- hancing effect and also improve image quality.