牛结核分枝杆菌溶血磷脂酶真核表达载体的构建及在Hela细胞中的表达

Construction of eukaryotic expression vector Lip-pEGFP and its expression in Hela cells

为了研究牛结核分枝杆菌溶血磷脂酶基因(Lip)的生物学特性及其功能,试验构建了Lip-pEGFP融合基因的真核表达载体,利用脂质体(lipofectamine2000)将其转染到Hela细胞中,用荧光显微镜检测GFP在细胞中的表达情况。结果显示,重组Lip-pEGFP载体在Hela细胞中获得了高效表达,且能够稳定传代,为进一步研究Lip的生物学功能及其在牛结核分枝杆菌中的作用奠定了基础。

In order to study the biological character and functions of the gene encoding lysophospholipase （Lip）of Mycobacterium bovis, the eukaryotic expression vector of fusion gene Lip-pEGFP was constructed. The vector was transfected into Hela cells by lipofectamine2000 and the expressed GFP protein was detected by fluorescence microscope. The results showed that the recombinant vector of Lip-pEGFP was highly expressed in Hela cells and can be stable expressed and passaged. This laid a foundation for further study on the biological functions of Lip and its effect on Mycobacterium boris.