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用卡片PCR法检测卡耶他环孢子虫 被引量：1

FTA-PCR method for detection of Cyclospora cayetanensis
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摘要 使用FTA卡片法提取卡耶他环孢子虫DNA,利用特异性引物组F1E/R2B、CC719/CRP999进行巢式PCR,得到1条298bp的特异条带。并以柔嫩艾美尔球虫、贝氏隐孢子虫等作为对照,结果显示,除卡耶他环孢子虫外,均无特异性条带。用卡耶他环孢子虫卵囊进行巢式PCR敏感性试验,结果显示敏感度最高达1.0×100个/mL。 DNA from Cyclospora cayetanensis （C. cayetanensis） was exracted by FTA,and used a nested PCR to amplify a 298-bp region of the small subunit rRNA gene with the unique primers of F1E/R2B and CC719/CRP999. DNA templates from Eirneria tenella and C. baileyi as control,only DNA template from C. cayetanensis produced an amplicon. 1.0×10^0 oocyst/mL could be detected by the multiplex PCR protocol.
作者 黄燕 张龙现 李晓虹 徐梅倩 吕彪 韩伟 朱顺海 周洋 曹杰 何国声
机构地区 中国农业科学院上海兽医研究所/农业部动物寄生虫学重点开放实验室 河南农业大学牧医工程学院 上海出入境检验检疫局
出处 《中国兽医学报》 CAS CSCD 北大核心 2010年第2期225-227,232,共4页 Chinese Journal of Veterinary Science
基金 中央级公益性科研院所基本科研业务费专项基金资助项目(shvri-2008JB04 2007JB07) 河南省高校人才培养工程资助项目(豫教高2004-294) 上海市科委标准专题资助项目(05dz05008)
关键词 卡耶他隐孢子虫 巢式PCR FTA Cyclospora cayetanensis nested-PCR FTA
分类号 S852.723 [农业科学—基础兽医学] R535 [医药卫生—内科学]
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参考文献9

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同被引文献10

  • 1Bereczky S,Martensson A,Gil J,A.Farnert. Short report:rapid DNA extraction from archive blood spots on filter for genotyping of Plasmodium falciparum[J].American Journal of Tropical Medicine and Hygiene,2005.249-251.
  • 2Huang S,Zhou X,Ren Z. Detection of β-thalassemia mutation in the Chinese using amplified DNA from dried blood specimens[J].Human Genetics,1990.129-131.
  • 3Panteleeff D,John G,Nduati G,Mbori-Ngacha D. Rapid method for screening dried blood samples on filter paper for diagnostic PCR using filter paper and Chelex-100[J].Molecular Pathology,1998.215-217.
  • 4Zhou H,Hickford J,Fang Q. A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification[J].Analytical Biochemistry,2006.159-161.
  • 5C.Régnier,O.Gargominy,G,Falkner. Foot mucus stored on FTA card is a reliable and non-invasive source of DNA for genetics studies in mollusks[J].Conservation Genet Resour,2011.377-382.
  • 6Andrew M.Borman,Mark Fraser,Christopher J. An improved protocol for the preparation of total genomic DNA from isolates of yeast and mould using whatman FTA filter papers[J].Mycopathologia,2010.445-449.
  • 7Karthipan S,George E,Jameela S. An assessment of three noncommercial DNA extraction methods from dried blood spots for b(e)te-thalassaemia mutation identification[J].International Journal of Laboratory Hematology,2011.87-92.
  • 8付素兰,刘景武,任天红,王国文.FTA卡在模板DNA制备中的应用研究[J].中国卫生检验杂志,2008,18(6):1124-1125. 被引量：9
  • 9鲁巧云,余进,刘伟,杨建勋,马蕾,李若瑜.FTA-DNA直接提取法在病原真菌分子鉴定中的应用[J].中国真菌学杂志,2010,5(3):137-140. 被引量：9
  • 10徐飞,成述儒,罗玉柱.FTA卡和普通定性滤纸提取DNA方法研究[J].生物技术通报,2011,27(11):221-224. 被引量：4

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中国兽医学报
2010年 第2期

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