结核性胸膜炎病变局部B淋巴细胞CD23、CD40表达及其影响因素的探讨

Expression of CD23、CD on B lymphocyte and it's influential factorsat the site of disease in patients with tuberculous pleuritis

采用细胞─酶联标记法测定了结核性胸膜炎病人胸水中Ｂ淋巴细胞ＣＤ２３、ＣＤ４０的表达，并进一步观察了Ｂ淋巴细胞在Ｔ淋巴细胞、ＰＰＤ等参与培养后ＣＤ２３、ＣＮ４０的表达受影响情况。结果显示：结核性胸膜炎病人胸水中Ｂ琳巴细胞与Ｔ淋巴细胞混合培养后与单独Ｂ淋巴细胞培养组相比较，ＣＤ２３的表达明显降低（Ｐ＜０．０５），二者存在密切相关（ｒ＝０．８０），而Ｂ＋ＰＰＤ、Ｂ＋ＰＰＤ＋Ｔ培养组与Ｂ淋巴细胞培养组相比较，ＣＤ２３表达无明显差异（Ｐ＞０．０５）；各组之间ＣＤ４０的表达无差异。健康人外周血Ｂ淋巴细胞培养过程中ＣＤ２３、ＣＤ４０的表达不受Ｔ细胞及ＰＰＤ的影响。提示：结核病人Ｔ细胞对Ｂ细胞ＣＤ２３的表达具有明显抑制作用。

To observe the activation of B lymphocytes in tuberculosis pleural fluids and the effects of T lymphocytes and PPD on the expression.T.B.cells were separated from pleural fluids and were cultured alone or mixed.Expression of CD23、Cd40 on B cells were determined by method of ELISA-CELL.The results showed Compared with B groups,expres- sion of CD23 on B+T groups were decreased(P<0.05),there was a close correlation between them(r=0.80);No dif- ferences were found between B+PPD groups and B groups、B+PPD+T groups and B groups.The CD expression on B cells affected by T cells are limited.There were no obvious effects of cells and PPD to the CD23、CD expression on B cells from PBMCs in controls.The results suggest,there are obvious inhibitory effects of T cells to the expression of CD on B cells in tuberculous patients.