化疗药物对肺癌细胞凋亡诱导作用的研究

A study of lung cancer cell apoptosis induced by Vp 16 and Delisheng

目的探讨化疗药物鬼臼乙叉甙（ｅｔｏｐｏｓｉｄｅ，Ｖｐ１６）及中成药得力生对肺癌细胞凋亡的诱导作用。方法采用电镜、ＤＮＡ电泳、ＴＵＮＥＬ原位末端标记和流式细胞术（ＦＣＭ）检测凋亡细胞。结果Ｖｐ１６及得力生在一定的作用时间与剂量下均能诱导小细胞肺癌和腺癌细胞的凋亡，凋亡细胞有典型的形态学改变及ＤＮＡ梯形带形成，ＦＣＭ检测凋亡细胞处于低ＤＮＡ含量位置（ＰｒｅＧ１峰），ＴＵＮＥＬ荧光染色能将凋亡细胞的形态与定量计数结合起来。结论Ｖｐ１６及得力生均能成功诱导肺癌细胞凋亡。

Objective To detect the lung cancer cell apoptosis induced by Vp 16 and a chinese herbal medication Delisheng. Methods Morphology, DNA gel electrophoresis, terminal deoxynucleotidyl transferase nick end labelling (TUNEL) and flowcytometric (FCM) DNA alalysis were used. Results Vp 16 and Delisheng could induce apoptosis on small cell lung cancer (SCLC) and adenocarcinoma cells. The apoptotic cells were charaterized by typtical morphologic changes, DNA “ladder” formation in gel electrophoresis, TUNEL positive cells and Pre G 1 peak in the FCM DNA histogram. Conclusion Vp 16 and Dlisheng could induce apoptosis on SCLC and adenocarcinoma cells and this induction is dose and time dependent to the chemotherapeutic agents.