青蒿琥酯对实验性免疫性肌炎动物模型的影响

Effect of Artesunate on Experimental Immune Myositis Animal Model

目的 建立类似于人类多发性肌炎 /皮肌炎 (PM /DM )的豚鼠模型 ,观察青蒿琥酯对此模型的影响。方法 用兔肌匀浆加完全佛氏佐剂免疫注射豚鼠 ,造成实验性免疫性肌炎 (EIM )动物模型 ,将EIM豚鼠随机分为青蒿琥酯治疗组和生理盐水对照组进行对比观察。结果 治疗组血清中肌酸激酶 (CPK )、乳酸脱氢酶 (LDH)、天冬氨酸转氨酶 (AST)较对照组显著降低。肌电图显示治疗组较对照组波幅明显增高 ,时限显著延长 ,但多相波阳性率无明显改变。肌肉病理可见治疗组较对照组在炎性细胞浸润 ,肌纤维断裂 ,坏死程度方面明显减轻。结论 本实验所建立的EIM豚鼠模型与人类PM相似 ,青蒿琥酯对EIM豚鼠有治疗作用 ,为今后临床上使用青蒿琥酯治疗PM

Objective To investigate the effect of Artesunate (Art) on experimental immune myositis (EIM) animal model. Methods Guinea pigs were immunized with rabbit muscle homogenate in Freund′s adjuvant, and a generalized myositis fundamentally similar to human polymyositis was established. These animals were divided into two groups: the control group (injected intraperitoneally with normal saline everyday) and the treatment group (injected intraperitoneally with Art everyday). Results The serum levels of CPK, AST and LDH were significantly lower in the treatment group than those in the control group. EMG demonstrated that the treatment group kept improving along with the elapse of time. Histological findings revealed that the damage of muscle in the treatment group was less severe than that of the control group. Conclusion Art is effective in therapy of EIM guinea pigs. The result of this study provides a reliable evidence that Art might be used for the treatment of PM/DM and other immune diseases.