摘要
用牛血清白蛋白为手性选择剂,异丙醇为修饰剂,应用毛细管电泳法拆分氧氟沙星对映体,实验时柱温为30℃,电泳电压15kV,采用50mg·ml-1牛血清白蛋白—5%异丙醇—磷酸盐缓冲液(pH60)为电泳电解液,检测波长为293nm,得到了良好拆分结果。左、右旋成分迁移时间及峰面积的RSD分别为16%,18%和11%,28%。应用于氧氟沙星产品研究,其左、右旋成分峰面积比值为0995~0998,RSD为18%~20%。并研究了pH值、牛血清白蛋白浓度、异丙醇浓度、柱温、电泳电压对对映体迁移时间及分辨率的影响。
An analytical method for separation of ofloxacin enantiomers by HPCE using bovine serum albumin(BSA) as the chiral selector and isopropanol as the modifier was established. The effects of BSA concentration, pH of running buffer, isopropanol concentration, applied voltage and column temperature on the resolution of the enantiomers have been studied. The experimental results indicated that the BSA concentration and pH of the running buffer were the vital factors for the separation of the enantiomers. The concentration of isopropanol showed strong influence on the peak shape of enantiomers. Lower voltage and column temperature were favorable to improve the effieiency for the separation of the enantiomers. Optimum performance was achieved in a fusedsilica capillary(45 cm×30 μm ID, effective length 32 cm) at 30℃ and applied voltage of +15 kV with a pH 60 phosphate buffer containning 50 mg·ml-1 BSA and 5% isopropanol as running buffer and detected at 293 nm. The precison of this method was evaluated by repeated measurement of the migration times and peak areas for 045 mg·ml-1 ofloxacin standard solution. The RSD(n=8) for migration times and peak areas were 16% and 11% for (-)ofloxacin and were 18% and 28% for (+)ofloxacin, respectively. The method was simple and fast and has been applied to separate enantiomers of ofloxacin products. The experimental results showed that the ratio of peak area of (-)ofloxacin to that of (+)ofloxacin was within the range of 0995~0998 with RSD(n=6) of measurement between 18% and 20%.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第8期600-604,共5页
Acta Pharmaceutica Sinica
关键词
毛细管电泳法
氧氟沙星
对映体
High perform capillary electrophoresis
Ofloxacin
Enantiomers