醚磺隆在稻株、稻米、土壤和田水中残留的分析方法

Analytical method for cinosulfuron residues in rice plant,rice,soil and water

研究并建立了醚磺隆（cinosulfuron）在水稻植株、稻米、土壤及田水中的残留分析方法。水稻植株和稻米样品以甲醇提取，经石油醚、二氯甲烷液液分配，固相萃取（SPE）净化，高效液相色谱（HPLC）测定；土壤样品经甲醇提取后，高效液相色谱（HPLC）测定；田水样品经固相萃取（SPE）净化，高效液相色谱（HPLC）测定。醚磺隆的最小检测量为2ng，水稻植株和稻米中醚磺隆最小检测浓度为0．04mg·kg^-1；土壤中醚磺隆最小检测浓度为0．02mg·kg^-1；田水中醚磺隆的最小检测浓度为0．04mg·L^-1。添加浓度0．04—0．2mg·kg^-1时，水稻植株中醚磺隆的回收率为98．1％～105．9％，变异系数3．9％-4．4％；稻米中醚磺隆的回收率为87．8％～98．8％，变异系数2．9％～6．6％；添加浓度0．02～0．2mg·kg^-1时，土壤中醚磺隆的回收率为87．9％～100．5％，变异系数2．5％～7．3％；添加浓度0．04～0．2mg·L^-1时，田水中醚磺隆的回收率为86．2％～107．9％，变异系数为3．3％～8．6％。该方法的准确性、精确性以及灵敏度均符合农药残留分析的要求。

SPE-HPLC was used to analyse residue of cinosulfuron in rice plant , rice, soil and water. Residues of cinosulfuron were extracted from rice plant , rice and soil with methanol, cleaned up by SPE cartridge, and determined by HPLC equipped with UV detector. The average recoveries and coefficients of variation of the method were 98.09% ～105.96% ,3.99% ～4.36% for rice plant samples;87.76% ～98.75% ,2.88%～6. 75% for rice samples; 87.85% ～ 100. 53% ,2.45% ～7.26% for soil samples; 86. 19% ～ 107.90% ,3.31% ～8.59% for water samples, respectively. The minimum detectable amount of cinosulfuron was 2ng, and the minimum detectable concentrations was 0. 04,0.02 mg· kg^-1 and 0. 04 mg·L^-1,respectively. The results showed that the method meet with demands of pesticide residue analysis.