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群落分析中的16S rRNA及其基因16S rDNA优化扩增 被引量：21

16S rRNA gene in community structure analysis and the optimized amplification-A review
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摘要 本文较为完整地综述了16SrRNA在细胞中的地位和作用、转录组成、其全长基因中可变区与保守区的位置、在不同种类中的拷贝数、二级结构,及其在群落研究过程中PCR扩增产生的16S rRNA的异常序列与解决方法;以期为更多的科研工作者提供可靠参考和借鉴,提高以16S rRNA为靶分子的群落研究结果的准确性和真实性。 The purpose of this review was to offer reference for the study of community analysis in which 16S rRNA was taken as target molecule. We summarized the information of 16S rRNA including its function in cells, transcription organization, the position of variable regions and conserved regions, copies numbers of 16S rRNA gene, secondary structure, some artifacts generated during PCR amplification and the solution to avoid the artifacts.
作者 周琳 张杰
机构地区 中国农业科学院植物保护研究所
出处 《微生物学报》 CAS CSCD 北大核心 2010年第1期7-14,共8页 Acta Microbiologica Sinica
基金 国家"973项目"(2007CB109203)~~
关键词 16S RRNA 可变区 保守区 异常序列 16S rRNA variable region conserved region artifacts
分类号 Q93 [生物学—微生物学]
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参考文献55

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微生物学报
2010年 第1期

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