耕作和施肥对不同深度黑土中细菌群落结构的影响

Effect of tillage and fertilization on bacterial community structure in different depth of black soil

【目的】为探讨耕作和施加有机肥、化肥对黑土表层(0-30cm)、中层(100-130cm)及深层(200-230cm)细菌群落结构的影响,【方法】应用DGGE技术对相应土层中细菌群落结构进行了解析。【结果】结果表明,与对照相比,耕作和施加有机肥、化肥对表层黑土细菌群落结构影响较大,二者差异度为4%;而对中层和深层细菌群落结构影响较小,二者差异度为2%。对于细菌群落结构的垂向变化,中层和深层中细菌群落结构的相似性远远高于同表层的相似性。【结论】可见,耕作和施加有机肥、化肥仅对黑土表层土壤(0-30cm)具有一定的影响,而对100cm以下土壤细菌群落影响较小,细菌群落随土壤深度不同的垂向变化要远高于土壤管理措施造成的影响。

[ Objective] To analyze the impact of tillage and fertilization on bacterial community structure under different depth in black soil. [ Methods] We used denaturing gradient gel electrophoresis （DGGE） profile analysis of bacterial community based on the 16S rRNA gene combining soil physico-chemical characteristics. [ Results] DGGE profiles showed that tillage and fertilization influenced more on bacterial community structrue in the surface soil than the subsurface and deep soil, and the divergence distance between the tillage soil and the control was about 4%. Subsurface and deep soil affected less compared with the control, and the divergence distance was 2%. The vertical similarity of bacterial community structure between subsurface and the deep soil was higher than that of suface soil. [ Conclusion ] Tillage and fertilization affected the surface bacterial community （0 -30 cm） , whereas little on the deeper soil （ 〉 100 cm） bacterial community.