SLE患者外周血CD4^＋T淋巴细胞DNA中p16基因启动子区甲基化研究

Methylation state of p16 gene promoter in peripheral CD4^＋ T cells of patients with systemic lupus erythematosus

目的检测SLE患者外周血CD4^＋T淋巴细胞DNA中p16基因的甲基化状态及其在SLE发病机制中的作用。方法以Taqman探针为基础的实时定量PCR（Methylight）方法检测28例SLE患者和20例正常人CD4^＋T淋巴细胞中p16基因启动子区甲基化状态。结果SLE患者CD4^＋T淋巴细胞的p16基因启动子区甲基化率（35．7％，10／28）高于对照组（10．0％，2／20）。两者比较差异有统计学意义（r=4．11，P〈0．05）。SLE患者疾病严重程度评分与对应的标准甲基化指数无统计学相关性（rs=-0．29，P〉0．05）。结论SLE患者CD4^＋T淋巴细胞p16基因甲基化异常，提示p16基因的高甲基化在SLE的发病中起一定作用。

Objective To detect the methylation status of p16 gene promoter in CD4＋ T cells of patients with systemic lupus erythematosus （SLE）, and its significance in the pathogenesis of SLE. Methods The p16 gene promoter methylation in peripheral CD4^＋ T cells was detected with the Taqman probe-based real- time PCR （Methylight） technology in 28 patients with SLE and 20 healthy human controls. Results The methylation rate of p16 gene promoter in peripheral CD4^＋ T cells was higher in patients with SLE than that in the controls （35.7% vs 10%,Х^2= 4.11, P 〈 0.05）. There was no correlation between SLE disease activity index （SLEDAI） and the normalized index of methylation （NIM） of p16 gene promoter （rs = -0.29, P 〉 0.05）. Conclusion The methylation status of p16 gene promoter is aberrant in CD4^＋ T cells of SLE patients, sug- gesting that the hypermethylation of p16 gene plays a certain role in the pathogenesis of SLE.