FHIT基因启动子甲基化及其蛋白表达与蕈样肉芽肿的关系

Fragile histidine triad （FHIT） gene promoter methylation and protein expression in mycosis fungoides

目的探讨蕈样肉芽肿中FHIT基因启动子甲基化程度及其蛋白表达情况。方法采用甲基化特异性PCR方法，测定48例蕈样肉芽肿组织标本FHIT基因启动子甲基化情况，免疫组化方法检测其蛋白表达情况。结果48例蕈样肉芽肿组织中有26例出现异常甲基化（54．2％），30例标本蛋白表达异常（63．5％）。FHIT基因甲基化情况与其蛋白表达异常之间有明显相关性，但FHIT基因甲基化与患者性别，疾病分期及是否有淋巴结扩散没有相关性。结论在蕈样肉芽肿中，FHIT基因的甲基化可能是其蛋白表达异常而失活的原因之一。

Objective To detect the FHIT gene promoter methylation and protein expression in mycosis fungoides （MF）. Methods Tissue specimens were collected from 48 patients with MF and 18 normal human controls. FHIT protein expression was determined by immunohistochemistry, and methylation status of FHIT gene by methylation-specific PCR. Results Abnormal methylation of FHIT gene was found in 26 （54.2%） out of the 48 specimens. Thirty （63.5%） specimens of MF were negative for FHIT protein, which was observed in all the control specimens. The promoter methylation of FHIT was closely correlated with the protein expression of FHIT, but unrelated to the sex of, tumor staging or lymph node metastasis in patients with MF. Conclusion The FHIT gene promoter methylation may contribute to the inactivation and abnormal expression of FHIT protein in MF.