稻绿核菌(稻曲病菌)分离方法的比较研究

Comparison of isolation methods for Ustilaginoidea virens,the pathogen of rice false smut

作者对不同情况下稻曲病菌的分离方法进行了比较研究。结果表明成熟早期稻曲球上的绝大多数新鲜的厚垣孢子具有萌发能力,及时进行分离培养是病原菌成功分离的关键。随保存时间的延长,厚垣孢子萌发能力急剧下降;消毒处理可杀死大部分的厚垣孢子。菌核可长期保存并保持极高的萌发生长能力,是稻曲病菌分离最为理想的材料。稻曲球中部的致密菌丝组织分离难度较大,只能作为稻曲病菌分离的一种补救方法。

Ustilaginoidea virens, the pathogen of rice false smut, was isolated from various diseased samples. The comparison of isolation methods showed that most fresh chlamydospores from earlier mature false smut balls could germinate and isolation time was the key for the successful isolation of the fungus. Prolongation of store period resulted in quick decline of the germination percentage of chlamydospores. Most of the chlamydospores could be killed by sterilizing treatment. It was proved that the sclerotium was the most ideal sample for isolation of the pathogen, which could keep alive for a very long time. However, the central dense hyphae in false smut balls were very difficult to grow into new colonies on medium and just an alternate method for isolation of the pathogen .